Limited Ability to Image Molecules in Their Native Contexts

Create whole-cell, nanometer-resolved, in-situ multi-omic imaging approaches that can map hundreds to thousands of molecules (e.g., proteins, RNAs) within intact specimens at nanoscale resolution. The core chemistries and imaging technologies for this exist, but they need to be integrated and brought to scale. We could, for example, comprehensively measure the many aspects of the “Hallmarks of Aging” within a single tissue sample.

Develop techniques for spatial multiplexing of dynamic signals in live cells, capturing real-time changes and molecular ticker-tapes that record cellular events over time.

Highly specific mAb/nanobody type binders for every target epitope, including for specific post-translational modifications

Develop methods to “freeze” and subsequently “unfreeze” living cells, effectively capturing dynamic states for later analysis and then resuming cellular function.

Develop live cell subcellular imaging techniques in tissues combined with computational foundation models to interpret the resulting data, enabling detailed mapping of subcellular structures and their dynamics  in native environments.